ABSTRACT
Objective:To explore effects of early long-term exercise training on learning and memory impairment induced by recurrent seizures in neonatal rats.Method:Eighteen Sprague-Dawley rats at postnatal days 8 (P8) were randomly divided into control group (CONT),convulsion group (EXP1),and convulsion plus physical exercise group (EXP2)(Six in each group).Rats were fed for 1 day before the study.From P9,rats in EXP1 and EXP2 were subjected to flurothyl induced recurrent seizures 30min daily for 7 days.The rats of EXP2 were physical exercise from P16 to P57.The rats in control group were placed into the container for an equal amount of time to their counterpart without exposing to flurothyl.On P58-P64,subjects of the three groups were tested with Morris water maze.Result:①Place navigation test:there were significant differences among three groups [F=429.90,P＜0.05],day [F=282.30,P＜0.05],interaction [F=12.71,P＜0.05] by repeated two-way ANOVA analysis.Compared with CONT group,the escape latency in EXP1 was longer (P＜0.05).Compared with EXP1,the escape latency in EXP2 was shorter (P＜ 0.05).②Spatial probe test:Compared with CONT group,the frequency of passing through the platform in EXP1 was much less (P＜0.05).While compared with EXP1,the frequency of passing through the platform in EXP2 was much more (P ＜ 0.05).Conclusion:Physical exercise could significantly reduce the escape latency time and increase the number of crossing the platform in the recurrent seizure group,which may be due to the improvement of learning and memory ability of convulsive rats by physical exercise.
ABSTRACT
The enantioseparations of 38 racemates on Chiralcel OD, Chiralpak AD, Chiralpak IA and(S, S)-Whelk-01 were presented by HPLC. Those enantiomers come from the amines, alcohols, ethers, ketones, aromatic derivatives, heterocyclic compounds, amide acids and medicines etc. With the mobile phase of n-hexane)/isopropanol(90∶ 10, V/V), n-hexane/isopropanol/trifluoracetic acid(90∶ 10∶ 0.2, V/V) or n-hexane/isopropanol/triethylamine(90∶ 10∶ 0.2, V/V), over 70% enantioseparations were obtained for OD, AD and IA columns). The order of enantioseparation selectivity for four columns was OD>AD>IA>(S,S)-Whelk-01, and among those columns there was a big chiral discriminating complementarity. This investigation was useful for choosing chiral columns to separate chiral compounds.